13083   Lum     Status: ES cell colonies screened / QC positives

GeneLum
Chromosome10
Deletion Start97,568,248
Deletion End97,572,137
Deletion Size3,890
Genome Build38
MGI IDMGI:109347
NCBI Gene ID17022
BAC ID211I7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,568,74297,568,763TCAACACAACCAGCTCAAAGAG
TUP97,568,76697,568,791TGCTGTCTCGGCTTCTCTGAAAGGTC
TUR97,568,80397,568,824TGAAGCTCAAATCCAGGTACTC
TDF97,571,92897,571,949GGGACCACTGTCTTACTCCAAG
TDP97,571,95297,571,975CAAGCATCTGCGCTTGGATGGCAA
TDR97,571,98897,572,008ATGTCAGGAGGCAGACTGCTC

PCR Primers

PrimerSequence
SUACATTGTGTGAGATGGTGCC
SDTGGTATCTTGCAAATTACTGGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR83 bp++-
TDFTDR81 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13083A-A5VGB6C57BL/6NTac
13083B-G8VGB6C57BL/6NTac
13083B-G10VGB6C57BL/6NTac
13083C-E2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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