| Gene | Sftpa1 |
| Chromosome | 14 |
| Deletion Start | 41,132,461 |
| Deletion End | 41,134,496 |
| Deletion Size | 2,036 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,132,472 | 41,132,490 | TTTGGCCTTCACCCTCTTC |
| TUP | 41,132,492 | 41,132,517 | TGACTGTTGTTGCTGGCATCAAGTGC |
| TUR | 41,132,525 | 41,132,544 | GCTTCCGGCACAAACTTCTG |
| TDF | 41,133,822 | 41,133,842 | GTGTGGACATAGGGCTTGGAG |
| TDP | 41,133,843 | 41,133,870 | AAAGGATTTCCTCCCGATGTCACTGGGA |
| TDR | 41,133,876 | 41,133,898 | TTATTGCAGGCATCCTCACTTTC |
PCR Primers
| Primer | Sequence |
| SU | AGGGACAGGTAGAGTAACCTGG |
| SD | GAAGAAGGGTTGAAGGGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13084A-B10 | VGB6 | C57BL/6NTac | |
| 13084A-C4 | VGB6 | C57BL/6NTac | |
| 13084A-D2 | VGB6 | C57BL/6NTac | |
| 13084A-D9 | VGB6 | C57BL/6NTac | |
| 13084A-G3 | VGB6 | C57BL/6NTac | |
| 13084A-F7 | VGB6 | C57BL/6NTac | |
