| Gene | P2rx3 |
| Chromosome | 2 |
| Deletion Start | 85,035,296 |
| Deletion End | 84,998,974 |
| Deletion Size | 36,323 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,035,104 | 85,035,123 | CCTCTCCCAAGGCTGCTCTG |
| TUP | 85,035,078 | 85,035,103 | CAGCTCCTAGACCCTCTTCTCTGGGA |
| TUR | 85,035,051 | 85,035,073 | GACATGGCTCCCACAAATCAAAG |
| TDF | 84,999,762 | 84,999,783 | TGAAGGTAGTCCCAGCCAAGAG |
| TDP | 84,999,735 | 84,999,760 | TAGGCAGTTAAGAGGCCATGTGAGCC |
| TDR | 84,999,684 | 84,999,707 | CTGGAGGAAGAGGAACAGCGTAAG |
PCR Primers
| Primer | Sequence |
| SU | GAGATGCCGCGTAAGAGAAC |
| SD | AGGGAAGGCAACTGACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13087A-B3 | VGB6 | C57BL/6NTac | |
| 13087A-C7 | VGB6 | C57BL/6NTac | |
| 13087A-C11 | VGB6 | C57BL/6NTac | |
| 13087A-F12 | VGB6 | C57BL/6NTac | |
| 13087A-D1 | VGB6 | C57BL/6NTac | |
