| Gene | Kcnj8 |
| Chromosome | 6 |
| Deletion Start | 142,570,376 |
| Deletion End | 142,565,536 |
| Deletion Size | 4,841 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,570,176 | 142,570,197 | CACCACCTTGGTAGACCTGAAG |
| TUP | 142,570,154 | 142,570,175 | TGGCGTCACACGCTGGTCATCT |
| TUR | 142,570,103 | 142,570,124 | GCCACCACATGATAGCGAAGAG |
| TDF | 142,566,481 | 142,566,500 | TCACGTCTGCGTTTCTCTTC |
| TDP | 142,566,453 | 142,566,480 | TCCATTGAGGTTCAAGTGACCATTGGGT |
| TDR | 142,566,422 | 142,566,443 | GGGCATTCCTCAGTCATCATTC |
PCR Primers
| Primer | Sequence |
| SU | CAGGCTTGCCTGACTAATCC |
| SD | TGCAGGACCTAAGAATGACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13090A-A2 | VGB6 | C57BL/6NTac | |
| 13090A-A11 | VGB6 | C57BL/6NTac | |
| 13090A-H10 | VGB6 | C57BL/6NTac | |
| 13090A-E7 | VGB6 | C57BL/6NTac | |
| 13090A-F4 | VGB6 | C57BL/6NTac | |
| 13090A-E11 | VGB6 | C57BL/6NTac | |
