| Gene | Tbx10 |
| Chromosome | 19 |
| Deletion Start | 3,993,111 |
| Deletion End | 3,999,337 |
| Deletion Size | 6,227 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,993,119 | 3,993,139 | GTATGGGTTCAGGCTCACAGG |
| TUP | 3,993,141 | 3,993,164 | CACTCACTCCTTGTGGGTCAGGGC |
| TUR | 3,993,204 | 3,993,224 | GTGCCACTGGACTGCTTATTC |
| TDF | 3,998,566 | 3,998,583 | TGAGGACCCAGCCCAAAC |
| TDP | 3,998,584 | 3,998,608 | ACAATGGCAGGCACATAGGCCTCCA |
| TDR | 3,998,617 | 3,998,635 | GTCGCCCATCGTGGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | AGTTTGGAGACACCCACCAG |
| SD | ACTGTGAGCCGAAACCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13099A-E5 | VGB6 | C57BL/6NTac | |
| 13099A-A4 | VGB6 | C57BL/6NTac | |
