| Gene | Cxcr3 |
| Chromosome | X |
| Deletion Start | 101,734,055 |
| Deletion End | 101,731,859 |
| Deletion Size | 2,197 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,733,882 | 101,733,905 | AAGAGCATTGGTGGATTGGGAAAC |
| TUP | 101,733,859 | 101,733,881 | CCAGCCTGGCCACTAACCACAGG |
| TUR | 101,733,837 | 101,733,857 | AGCCACAGAGGCAGGAGACAG |
| TDF | 101,732,535 | 101,732,554 | AGCCCTCACCTGCATAGTTG |
| TDP | 101,732,504 | 101,732,527 | TCTCTGTCTGCTCTTTGCCCTCCC |
| TDR | 101,732,464 | 101,732,483 | GAGGCGCTGATCGTAGTTGG |
PCR Primers
| Primer | Sequence |
| SU | TCCCAGTGAGACTCCAATCC |
| SD | ACTGTGAATACGCCCTGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13100A-B2 | VGB6 | C57BL/6NTac | |
| 13100A-D4 | VGB6 | C57BL/6NTac | |
| 13100A-E2 | VGB6 | C57BL/6NTac | |
| 13100A-F1 | VGB6 | C57BL/6NTac | |
| 13100A-G1 | VGB6 | C57BL/6NTac | |
| 13100A-G12 | VGB6 | C57BL/6NTac | |
