| Gene | Ltb4r1 |
| Chromosome | 14 |
| Deletion Start | 55,767,245 |
| Deletion End | 55,768,325 |
| Deletion Size | 1,081 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,767,298 | 55,767,317 | GTCTCTGTTGCCCATTGTTC |
| TUP | 55,767,318 | 55,767,340 | TACTGTCTGTGGCCCTGGCTGTG |
| TUR | 55,767,389 | 55,767,407 | CGGTGACCGTGCGTTTCTG |
| TDF | 55,767,913 | 55,767,934 | GGTGCTCATTATCCTGGCCTTC |
| TDP | 55,767,936 | 55,767,957 | CCGCCTTCTGGCTGCCTTACCA |
| TDR | 55,767,962 | 55,767,980 | CGGCTTCCACCAGGTTCAC |
PCR Primers
| Primer | Sequence |
| SU | TCTTTCTATTCAACCAGGCG |
| SD | GTGCTTCTCTAAGCGGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13101A-H7 | VGB6 | C57BL/6NTac | |
| 13101A-C12 | VGB6 | C57BL/6NTac | |
| 13101A-D1 | VGB6 | C57BL/6NTac | |
| 13101A-D3 | VGB6 | C57BL/6NTac | |
| 13101A-H2 | VGB6 | C57BL/6NTac | |
| 13101A-F5 | VGB6 | C57BL/6NTac | |
