| Gene | Gpr37 |
| Chromosome | 6 |
| Deletion Start | 25,689,093 |
| Deletion End | 25,669,039 |
| Deletion Size | 20,055 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,688,503 | 25,688,523 | TCAGCAGTACACGAAGGAAGG |
| TUP | 25,688,481 | 25,688,502 | ACCCTTGCTCCTCCAGGCAGGG |
| TUR | 25,688,439 | 25,688,457 | GGTCAGGAACCCAGTCATC |
| TDF | 25,669,736 | 25,669,754 | TCTTCCACCACTGCCAAAC |
| TDP | 25,669,712 | 25,669,735 | TTGCCGTTATCTGGGTTGGAGCTC |
| TDR | 25,669,676 | 25,669,694 | GCTGGCGGAGGACAACTTC |
PCR Primers
| Primer | Sequence |
| SU | TTTCAAGGTCAGCCTCTTGG |
| SD | TTTGGTGCTAAGTTGCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13102A-F6 | VGB6 | C57BL/6NTac | |
| 13102A-H8 | VGB6 | C57BL/6NTac | |
| 13102A-F12 | VGB6 | C57BL/6NTac | |
| 13102A-C4 | VGB6 | C57BL/6NTac | |
