| Gene | Atp6v0e |
| Chromosome | 17 |
| Deletion Start | 26,676,478 |
| Deletion End | 26,695,538 |
| Deletion Size | 19,061 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,677,003 | 26,677,023 | GTTCAGCCTTTGCAGCTAGTG |
| TUP | 26,677,026 | 26,677,047 | CCCAACCCAAGCCAGGGTGAGC |
| TUR | 26,677,107 | 26,677,125 | GAGCGCCCTGGAAGGTATG |
| TDF | 26,695,433 | 26,695,453 | GTACCACTGGCCTTGAGGATG |
| TDP | 26,695,454 | 26,695,475 | AAGACGCGCTGCACAGTGCCTA |
| TDR | 26,695,511 | 26,695,532 | AGCTGACTGGCTGTCAATGTTC |
PCR Primers
| Primer | Sequence |
| SU | AAGACTCTAGAACAGTAGTCC |
| SD | TCTAATCTGCTGGGAGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13104A-C10 | VGB6 | C57BL/6NTac | |
| 13104A-F4 | VGB6 | C57BL/6NTac | |
