| Gene | Tpp1 |
| Chromosome | 7 |
| Deletion Start | 105,752,184 |
| Deletion End | 105,746,558 |
| Deletion Size | 5,627 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,751,974 | 105,751,993 | CCAGCGGTGGATGTGAGTTG |
| TUP | 105,751,945 | 105,751,969 | AGTACTACAGGTGCCTGTCTCTGCA |
| TUR | 105,751,906 | 105,751,929 | AGGAGCAGTTGAGGATGAGGAAAG |
| TDF | 105,746,768 | 105,746,789 | CCATCATCTCCTCCCAGGTAAC |
| TDP | 105,746,744 | 105,746,766 | CACGGCTGCCATGAGTCCTGTCT |
| TDR | 105,746,721 | 105,746,743 | CCTGACCCTCCACTTCTTCATTC |
PCR Primers
| Primer | Sequence |
| SU | AATATTGTACGGCCAGGAGC |
| SD | TTCAGTGCCGAGAGAAGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13108A-B7 | VGB6 | C57BL/6NTac | |
| 13108A-C1 | VGB6 | C57BL/6NTac | |
| 13108A-C4 | VGB6 | C57BL/6NTac | |
| 13108A-C11 | VGB6 | C57BL/6NTac | |
| 13108A-D9 | VGB6 | C57BL/6NTac | |
| 13108A-D12 | VGB6 | C57BL/6NTac | |
