| Gene | S1pr3 |
| Chromosome | 13 |
| Deletion Start | 51,418,788 |
| Deletion End | 51,420,021 |
| Deletion Size | 1,234 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,419,123 | 51,419,141 | CCTCAGGGAGGGCAGTATG |
| TUP | 51,419,142 | 51,419,162 | TTCGTAGCCCTGGGCGCATCC |
| TUR | 51,419,185 | 51,419,206 | ATGGTCAGGTGTCGCTCAATGG |
| TDF | 51,419,722 | 51,419,741 | TGTGCGGCTGTCTAGTCAAG |
| TDP | 51,419,751 | 51,419,770 | ACCCAGGCCTCACCCATGCA |
| TDR | 51,419,809 | 51,419,829 | GAGAGTGGCTGCTGTTGTTAC |
PCR Primers
| Primer | Sequence |
| SU | AACGCAGTATTTCCTGGTGC |
| SD | TTTGGACCAATGATGTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13109A-B2 | VGB6 | C57BL/6NTac | |
| 13109A-F1 | VGB6 | C57BL/6NTac | |
| 13109A-G1 | VGB6 | C57BL/6NTac | |
| 13109A-G3 | VGB6 | C57BL/6NTac | |
