| Gene | Nfe2l3 |
| Chromosome | 6 |
| Deletion Start | 51,432,910 |
| Deletion End | 51,458,454 |
| Deletion Size | 25,545 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,433,629 | 51,433,650 | GAAATCCATTGCGTGCAGAGAC |
| TUP | 51,433,651 | 51,433,678 | CACCCTTCCTTGTGTTTGATGGCTGTGG |
| TUR | 51,433,708 | 51,433,728 | ACGTCCAGACAGCACCATTCC |
| TDF | 51,457,925 | 51,457,948 | TGGATGCCTTGATAACCCAGATAG |
| TDP | 51,457,953 | 51,457,976 | TTGAGCCGTGATGAACAGCGTGCT |
| TDR | 51,457,999 | 51,458,023 | GGCATACGGACAATTTCATCTACAG |
PCR Primers
| Primer | Sequence |
| SU | AACTTTCCTCCACTCCCACC |
| SD | AAGGCAAGTATTCTTTGGACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13110A-B3 | VGB6 | C57BL/6NTac | |
| 13110A-E5 | VGB6 | C57BL/6NTac | |
