| Gene | Atrn |
| Chromosome | 2 |
| Deletion Start | 130,906,564 |
| Deletion End | 130,935,632 |
| Deletion Size | 29,069 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,907,078 | 130,907,096 | GCCTCCTGGAGGGATGTTC |
| TUP | 130,907,097 | 130,907,120 | ATTTGGTGGCCTTCGGGTCTGCCT |
| TUR | 130,907,130 | 130,907,151 | GTGTTCCCGTTCCTTCTCAATC |
| TDF | 130,934,980 | 130,935,000 | TGTGGAGTCAGAGGGAGTTAC |
| TDP | 130,935,005 | 130,935,031 | ACACTTGTTGAAATTGCCCAGGCTTCA |
| TDR | 130,935,059 | 130,935,077 | ACAAGCCCTGCCCAGAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGGCGTCCAAAGGGTAACAC |
| SD | GTTCAAGAATGTGAAATGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13112A-C12 | VGB6 | C57BL/6NTac | |
| 13112A-D4 | VGB6 | C57BL/6NTac | |
| 13112A-D7 | VGB6 | C57BL/6NTac | |
| 13112A-F4 | VGB6 | C57BL/6NTac | |
| 13112A-H11 | VGB6 | C57BL/6NTac | |
