| Gene | Grem1 |
| Chromosome | 2 |
| Deletion Start | 113,750,151 |
| Deletion End | 113,749,518 |
| Deletion Size | 634 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,749,930 | 113,749,949 | CTGGAGAGGAGGTGCTTGAG |
| TUP | 113,749,910 | 113,749,929 | TCCAGCCAAGAGGCCCTGCA |
| TUR | 113,749,888 | 113,749,908 | CAGATACTTGCGCTCTGTCAC |
| TDF | 113,749,726 | 113,749,746 | TCCTTTCAGTCTTGCTCCTTC |
| TDP | 113,749,700 | 113,749,725 | TGCAAGCCCAAGAAGTTCACCACCAT |
| TDR | 113,749,656 | 113,749,674 | TCTTGGTGGGTGGCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | CTCTTGGCCTTCCGTACATC |
| SD | GTTCCGAGTCTAATGCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13113A-D4 | VGB6 | C57BL/6NTac | |
| 13113A-D5 | VGB6 | C57BL/6NTac | |
| 13113A-H11 | VGB6 | C57BL/6NTac | |
| 13113B-A2 | VGB6 | C57BL/6NTac | |
| 13113B-B10 | VGB6 | C57BL/6NTac | |
| 13113B-D8 | VGB6 | C57BL/6NTac | |
