| Gene | Insl5 |
| Chromosome | 4 |
| Deletion Start | 103,026,689 |
| Deletion End | 103,018,062 |
| Deletion Size | 8,628 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,026,619 | 103,026,639 | GGAAGTAAGAAGCAGGCAGAC |
| TUP | 103,026,594 | 103,026,618 | TGTGAAGCTCTGTGGCCTGGACTAC |
| TUR | 103,026,557 | 103,026,576 | ACCGTGAGCTGGCACAGATG |
| TDF | 103,018,712 | 103,018,736 | GGGCTTGAATATAGACACTGGGAAC |
| TDP | 103,018,672 | 103,018,700 | TAAACAATGTGGCCCATGCTTCAGTATCA |
| TDR | 103,018,637 | 103,018,660 | GGCAGAGCTGATAAGTGGGATTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTGAGAAGATGCAGCAATG |
| SD | TCACAGTGAGTTTGGATTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13116B-A10 | VGB6 | C57BL/6NTac | |
| 13116A-B11 | VGB6 | C57BL/6NTac | |
