| Gene | Aplnr |
| Chromosome | 2 |
| Deletion Start | 85,136,636 |
| Deletion End | 85,137,804 |
| Deletion Size | 1,169 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,136,746 | 85,136,765 | TCTAGGCACCACAGGCAATG |
| TUP | 85,136,767 | 85,136,790 | CCTGGTGCTCTGGACCGTGTTTCG |
| TUR | 85,136,819 | 85,136,840 | CCAGGCTGGCAATGAAGATGTC |
| TDF | 85,137,335 | 85,137,354 | ATTGAGGGCCTGCGGAAGAG |
| TDP | 85,137,356 | 85,137,378 | CGCCGGCTGCTCAGCATTATCGT |
| TDR | 85,137,386 | 85,137,407 | AGCACAGGGCAAAGGTCACTAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTCAGCCCAGCACTGTAG |
| SD | GGGAAGGGACCCACTAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13117A-A3 | VGB6 | C57BL/6NTac | |
| 13117A-A8 | VGB6 | C57BL/6NTac | |
| 13117A-C6 | VGB6 | C57BL/6NTac | |
| 13117A-D3 | VGB6 | C57BL/6NTac | |
| 13117A-G2 | VGB6 | C57BL/6NTac | |
| 13117A-D2 | VGB6 | C57BL/6NTac | |
