| Gene | Scamp2 |
| Chromosome | 9 |
| Deletion Start | 57,561,057 |
| Deletion End | 57,587,581 |
| Deletion Size | 26,525 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,561,750 | 57,561,772 | GGTACACGGGTCTGACAAATCTG |
| TUP | 57,561,775 | 57,561,796 | TGGGTGGTTACCGGCCTGACCT |
| TUR | 57,561,852 | 57,561,872 | ACTGGGCATCTCTGCGTTCTC |
| TDF | 57,587,150 | 57,587,171 | GCTGGCTTCTTTACCCTCTGTG |
| TDP | 57,587,172 | 57,587,196 | CTGGGCTTTCACTCTTCCTCCTGCA |
| TDR | 57,587,201 | 57,587,223 | GCTGTTGGCTCTGTCCTACTTAC |
PCR Primers
| Primer | Sequence |
| SU | AATGCATGATCCTTTCCTGC |
| SD | CATGGAGCCTGTAGAGGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13119A-B7 | VGB6 | C57BL/6NTac | |
| 13119A-B8 | VGB6 | C57BL/6NTac | |
| 13119A-G4 | VGB6 | C57BL/6NTac | |
| 13119A-H6 | VGB6 | C57BL/6NTac | |
