| Gene | Vti1a |
| Chromosome | 19 |
| Deletion Start | 55,316,454 |
| Deletion End | 55,328,450 |
| Deletion Size | 11,997 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,317,259 | 55,317,280 | CGTACAGGACAACTTTGCTCAC |
| TUP | 55,317,286 | 55,317,315 | CAATTCAGCATCCTCCAGGTATTACCCTGC |
| TUR | 55,317,331 | 55,317,350 | AGGCTCCCACCTTCCCACTG |
| TDF | 55,327,724 | 55,327,745 | CCAAAGCGTTACAGGATGACAG |
| TDP | 55,327,747 | 55,327,775 | TGGCTAAAGCATGCCCTATCGTGATTTCG |
| TDR | 55,327,776 | 55,327,793 | TAGCCACACCCTGCTCAC |
PCR Primers
| Primer | Sequence |
| SU | GTGATACCCGAGAGCATGTG |
| SD | CAGGAACTATAAAGGACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13121A-D11 | VGB6 | C57BL/6NTac | |
| 13121A-F5 | VGB6 | C57BL/6NTac | |
