| Gene | Celsr2 |
| Chromosome | 3 |
| Deletion Start | 108,415,491 |
| Deletion End | 108,392,575 |
| Deletion Size | 22,917 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,415,138 | 108,415,157 | GCTGTCGTCTCCTGGGTATC |
| TUP | 108,415,108 | 108,415,132 | ACACCTTTCTCCACAAGGCACGCTG |
| TUR | 108,415,079 | 108,415,100 | TTTAAGCAAGGGTGCTCTTCAG |
| TDF | 108,392,724 | 108,392,745 | CTGGTGGAAGCTGTTCACTGTC |
| TDP | 108,392,699 | 108,392,723 | CCCTCTGTTGCTCCAGCTAACCCTC |
| TDR | 108,392,678 | 108,392,697 | GAGTGGCAGAGAAGGCAGAC |
PCR Primers
| Primer | Sequence |
| SU | CGCAGGTAGAGAACGCTCAG |
| SD | CTGAAGAGAGGCTGGGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13122A-B8 | VGB6 | C57BL/6NTac | |
| 13122A-F3 | VGB6 | C57BL/6NTac | |
| 13122A-G10 | VGB6 | C57BL/6NTac | |
