| Gene | Vamp5 |
| Chromosome | 6 |
| Deletion Start | 72,380,380 |
| Deletion End | 72,369,252 |
| Deletion Size | 11,129 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,379,461 | 72,379,481 | CGAGAGTACAGGGCAGAGATG |
| TUP | 72,379,431 | 72,379,457 | TTTCAACTGGCTCTCAGCTCTTGAGGA |
| TUR | 72,379,383 | 72,379,404 | TGAACAGCCAGATAACCTAACC |
| TDF | 72,370,070 | 72,370,089 | GGCTTGCCTGCTTATCAGTG |
| TDP | 72,370,043 | 72,370,069 | TGCAACTTCCGGGTTTCCTACAAGTCG |
| TDR | 72,370,021 | 72,370,042 | CCCAAAGGCATCTTGGTGAAAC |
PCR Primers
| Primer | Sequence |
| SU | AGACGCACTCACCAAGGTG |
| SD | TTTACTGAGCTCCAGTGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13123A-E2 | VGB6 | C57BL/6NTac | |
| 13123A-E5 | VGB6 | C57BL/6NTac | |
| 13123A-F3 | VGB6 | C57BL/6NTac | |
| 13123A-G9 | VGB6 | C57BL/6NTac | |
