| Gene | Icos |
| Chromosome | 1 |
| Deletion Start | 60,977,962 |
| Deletion End | 60,997,731 |
| Deletion Size | 19,770 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,978,150 | 60,978,173 | AACAAGCACAGACAGTTTCAGAGG |
| TUP | 60,978,175 | 60,978,198 | ACTGAAGCTCACGGCTCAGGGCGT |
| TUR | 60,978,206 | 60,978,225 | CACTGACATCCACGCTGACC |
| TDF | 60,997,451 | 60,997,475 | CAACACATACCCATGAGAGTCTTAC |
| TDP | 60,997,477 | 60,997,501 | TGACTGCATCTGGACCCTGTGATGG |
| TDR | 60,997,512 | 60,997,535 | GTGAGGACTGGACATTATCTGAAC |
PCR Primers
| Primer | Sequence |
| SU | AAGCACAAATGACACATAAATAGC |
| SD | AAATGAGGACAGATGGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13124A-A10 | VGB6 | C57BL/6NTac | |
| 13124A-G4 | VGB6 | C57BL/6NTac | |
