| Gene | Cacna1f |
| Chromosome | X |
| Deletion Start | 7,607,149 |
| Deletion End | 7,635,138 |
| Deletion Size | 27,990 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,607,861 | 7,607,881 | ACCACAGAGCCAATGCTCCAG |
| TUP | 7,607,884 | 7,607,911 | TGAGCAGACCCTTCCTTCTACAAACACA |
| TUR | 7,607,948 | 7,607,966 | GAGCCTCCGCAGCTTACAG |
| TDF | 7,634,702 | 7,634,721 | TGAGAGCCGAGGATCAAGAG |
| TDP | 7,634,722 | 7,634,748 | TGGCCAGACTCTATGTTGAGCATGTGG |
| TDR | 7,634,755 | 7,634,779 | GTCAAGTACAGTATGCCGAGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CACTTAACCAGTGCCTTTCC |
| SD | TCCTCTTATCTGCCCAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13126A-A8 | VGB6 | C57BL/6NTac | |
| 13126A-B3 | VGB6 | C57BL/6NTac | |
| 13126A-B5 | VGB6 | C57BL/6NTac | |
| 13126A-F8 | VGB6 | C57BL/6NTac | |
| 13126A-G5 | VGB6 | C57BL/6NTac | |
