| Gene | Fxyd4 |
| Chromosome | 6 |
| Deletion Start | 117,935,890 |
| Deletion End | 117,933,723 |
| Deletion Size | 2,168 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,935,837 | 117,935,857 | GCAGGTGAGTTCTCCATACAC |
| TUP | 117,935,813 | 117,935,835 | CCGCCCACCAAGGTAGTGGAGAC |
| TUR | 117,935,774 | 117,935,793 | TTATTGGGCCGCAGTCCTAC |
| TDF | 117,934,308 | 117,934,328 | ACCCATAAGCCCAGGTAAGAC |
| TDP | 117,934,276 | 117,934,302 | CCAAGTGGTACGGATCTTTGGCTGAGC |
| TDR | 117,934,224 | 117,934,245 | TCAGGGTGTAGTGGTTCTGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGCCTACGTGGTCACTCCAG |
| SD | GGAGAGCATTGGAGGCATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13130A-A5 | VGB6 | C57BL/6NTac | |
| 13130A-A10 | VGB6 | C57BL/6NTac | |
| 13130A-D8 | VGB6 | C57BL/6NTac | |
| 13130A-G8 | VGB6 | C57BL/6NTac | |
| 13130A-G11 | VGB6 | C57BL/6NTac | |
