13132   Il21r     Status: ES cell colonies screened / QC positives

GeneIl21r
Chromosome7
Deletion Start125,622,885
Deletion End125,632,991
Deletion Size10,107
Genome Build38
MGI IDMGI:1890475
NCBI Gene ID60504
BAC ID455B9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,623,606125,623,626TGTCATCTCCTGCTCTTCCTG
TUP125,623,627125,623,649TGGAGCCTGGCTGTGTTGCCATC
TUR125,623,651125,623,671CCTGAACCTCTGTCTTTCCAC
TDF125,632,354125,632,376TGTATCCAGCCAAGGAGAAGAAG
TDP125,632,386125,632,408CTGCCGGGTCTGGAAGAGCAACT
TDR125,632,414125,632,435CCAGGCTCAGACATTCCATCAC

PCR Primers

PrimerSequence
SUTCAGCAACTTTGTGTGGTGC
SDGATGTGCTAACACACAACCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR66 bp++-
TDFTDR82 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13132A-D6VGB6C57BL/6NTac
13132A-F3VGB6C57BL/6NTac
13132A-F5VGB6C57BL/6NTac
13132A-G9VGB6C57BL/6NTac
13132A-G8VGB6C57BL/6NTac
13132A-F8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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