| Gene | Ccbp2 |
| Chromosome | 9 |
| Deletion Start | 121,908,564 |
| Deletion End | 121,909,725 |
| Deletion Size | 1,162 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,908,796 | 121,908,818 | CGATGGAGCTTTACCTGCTGAAC |
| TUP | 121,908,820 | 121,908,846 | TGGCCGTCTCCAACCTCTTGTTTGTAG |
| TUR | 121,908,866 | 121,908,886 | CAATGCCAGGCCACAGAGATG |
| TDF | 121,909,482 | 121,909,501 | CCGGTCCTCTACGCCTTCTG |
| TDP | 121,909,502 | 121,909,523 | CAGTCACCGCTTCCGCCGGTAC |
| TDR | 121,909,547 | 121,909,565 | TGCCTGGTGCCATCTCAAC |
PCR Primers
| Primer | Sequence |
| SU | CTCACCTCAGGCATTTAGCC |
| SD | TTGAAGGTCTGAAGGCTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13133A-A5 | VGB6 | C57BL/6NTac | |
| 13133A-B2 | VGB6 | C57BL/6NTac | |
| 13133A-B8 | VGB6 | C57BL/6NTac | |
| 13133A-B11 | VGB6 | C57BL/6NTac | |
| 13133A-D4 | VGB6 | C57BL/6NTac | |
| 13133A-D8 | VGB6 | C57BL/6NTac | |
