| Gene | Cox16 |
| Chromosome | 12 |
| Deletion Start | 81,484,962 |
| Deletion End | 81,472,159 |
| Deletion Size | 12,804 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,484,920 | 81,484,941 | GCTCTGCGTAAGAACAAGACTC |
| TUP | 81,484,893 | 81,484,918 | ACGCTATGGAGTTCCCATGTTGGTGA |
| TUR | 81,484,824 | 81,484,844 | CAATACGCCACTCAGCCAAAG |
| TDF | 81,472,473 | 81,472,492 | CCTGAGGTTGGCTTGGAGTC |
| TDP | 81,472,440 | 81,472,468 | TGCTGAACTTGTTCTTGAGCCTATTGGGT |
| TDR | 81,472,387 | 81,472,408 | AGCCCAGTGGCTTTGAGTGATG |
PCR Primers
| Primer | Sequence |
| SU | TCGCAGAAGTCAAGATCCAG |
| SD | TGAACAGGTTTCAGAGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13136A-A10 | VGB6 | C57BL/6NTac | |
| 13136A-D3 | VGB6 | C57BL/6NTac | |
| 13136A-F11 | VGB6 | C57BL/6NTac | |
| 13136A-G12 | VGB6 | C57BL/6NTac | |
| 13136A-F9 | VGB6 | C57BL/6NTac | |
| 13136A-G11 | VGB6 | C57BL/6NTac | |
