| Gene | Spcs2 |
| Chromosome | 7 |
| Deletion Start | 99,858,874 |
| Deletion End | 99,839,591 |
| Deletion Size | 19,284 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,858,551 | 99,858,571 | CAAGTGCTGCTGGTCCCTAAC |
| TUP | 99,858,524 | 99,858,550 | CGCCACCGCAGCTTCTCGTAATTATGG |
| TUR | 99,858,476 | 99,858,496 | GTGGCCCTCCTAGCACAATAG |
| TDF | 99,840,369 | 99,840,389 | GGATAGCCTGGTGTTCTCATC |
| TDP | 99,840,339 | 99,840,367 | TCATTAGGGTTCAGCAGTTAAACCAGGGA |
| TDR | 99,840,268 | 99,840,288 | CCCACTCACAGGTCTCCTAAC |
PCR Primers
| Primer | Sequence |
| SU | AGCAGGCTCTACCTCCCTTC |
| SD | TGCATGGCACAAAGCTAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13138A-D6 | VGB6 | C57BL/6NTac | |
| 13138A-H7 | VGB6 | C57BL/6NTac | |
| 13138A-B5 | VGB6 | C57BL/6NTac | |
