| Gene | Rnf128 |
| Chromosome | X |
| Deletion Start | 139,610,809 |
| Deletion End | 139,642,527 |
| Deletion Size | 31,719 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,611,223 | 139,611,240 | GCTTCTGGAGCGGTCATC |
| TUP | 139,611,243 | 139,611,267 | TAACTTCCCTGGGACCCGCAATGAG |
| TUR | 139,611,289 | 139,611,311 | GCAAATTAGTAGCTGCACTCACC |
| TDF | 139,641,755 | 139,641,774 | GGAGGATCAGGCAGAAGGAC |
| TDP | 139,641,778 | 139,641,801 | AGTTTCAGGCTTGCCTGGGATGCA |
| TDR | 139,641,808 | 139,641,827 | TGGAGTCTAGGTTGCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | CGCAGTGTCCTTTTCCTCTC |
| SD | ACATTTACCTTCAGGCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13139A-C6 | VGB6 | C57BL/6NTac | |
| 13139A-C10 | VGB6 | C57BL/6NTac | |
