Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

13142 Trit1 Status: ES cell colonies screened / QC positives

Gene	Trit1
Chromosome	4
Deletion Start	123,016,613
Deletion End	123,054,294
Deletion Size	37,682
Genome Build	38

MGI ID	MGI:1914216
NCBI Gene ID	66966
BAC ID	49H8
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	123,016,902	123,016,922	CCAGATGAAAGGAACCCACAC
TUP	123,016,927	123,016,954	TAGGTACCCATTCCAATGGCAGACAGTG
TUR	123,016,957	123,016,978	TTAGCGATCACAGGACACAATG
TDF	123,053,861	123,053,879	CCTGCTGGCAGAGCCTTAG
TDP	123,053,881	123,053,906	CAATGTACAGCACAGGCCTAGAGCCG
TDR	123,053,941	123,053,961	TACTAGGTAGCCCACACTGAC

PCR Primers

Primer	Sequence
SU	GGCAAGTCGTTTCTCTGTCC
SD	CCTGACTGCGCCTTACAATAC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	77 bp	+	+	-
TDF	TDR	101 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
13142A-B3	VGB6	C57BL/6NTac
13142A-H7	VGB6	C57BL/6NTac
13142A-A12	VGB6	C57BL/6NTac