| Gene | Erp29 |
| Chromosome | 5 |
| Deletion Start | 121,452,337 |
| Deletion End | 121,445,027 |
| Deletion Size | 7,311 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,451,513 | 121,451,535 | GCAAATGGACGGTGGAAATGTTG |
| TUP | 121,451,491 | 121,451,512 | CAGGCTGAGTGCAGTGGCCTCT |
| TUR | 121,451,425 | 121,451,449 | CGCTAAGCCCAATAGAGGAACTAAG |
| TDF | 121,445,346 | 121,445,365 | TGGCAGGCGAGTTCATCAAG |
| TDP | 121,445,323 | 121,445,344 | CCTCCAGCATAGAGGCCCGACA |
| TDR | 121,445,295 | 121,445,316 | GCCATCCTGTCCCTGTTTCAAG |
PCR Primers
| Primer | Sequence |
| SU | GAGAGAGAGGGCAACGAGG |
| SD | GAAACGTAAGGCTGTGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 111 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13147B-E5 | VGB6 | C57BL/6NTac | |
| 13147B-G10 | VGB6 | C57BL/6NTac | |
| 13147C-G1 | VGB6 | C57BL/6NTac | |
| 13147C-G3 | VGB6 | C57BL/6NTac | |
