| Gene | Tomm20 |
| Chromosome | 8 |
| Deletion Start | 126,945,721 |
| Deletion End | 126,934,919 |
| Deletion Size | 10,803 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,944,880 | 126,944,898 | AAAGGGCTGCGCTCCTCAC |
| TUP | 126,944,850 | 126,944,878 | TGGCTCAGTCTTATTTCATACCTGCGACA |
| TUR | 126,944,807 | 126,944,826 | CCAAGGCGTGCCAAGATTTC |
| TDF | 126,935,234 | 126,935,256 | AGCGTCTCTAGTGCTGAGGTTTG |
| TDP | 126,935,208 | 126,935,233 | AGGTATGTGTCACCATGCCCACCTGA |
| TDR | 126,935,155 | 126,935,179 | CCCCATTTTGACATAGATCCCATAC |
PCR Primers
| Primer | Sequence |
| SU | GACTCAGCACGCCACAAAC |
| SD | CGAGAAACGGTCTCTGTAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13152A-B3 | VGB6 | C57BL/6NTac | |
| 13152A-C4 | VGB6 | C57BL/6NTac | |
| 13152A-D6 | VGB6 | C57BL/6NTac | |
| 13152A-E12 | VGB6 | C57BL/6NTac | |
| 13152A-F11 | VGB6 | C57BL/6NTac | |
