| Gene | Stoml1 |
| Chromosome | 9 |
| Deletion Start | 58,253,270 |
| Deletion End | 58,261,897 |
| Deletion Size | 8,628 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,253,590 | 58,253,610 | AATCTTAGCCCATCCCGTTAC |
| TUP | 58,253,615 | 58,253,643 | TCCAGGATAGCCCATCATGCATAGAAAGA |
| TUR | 58,253,659 | 58,253,678 | GTGGGTTATGGCTCCCTCTG |
| TDF | 58,261,448 | 58,261,467 | GCCCTGAGCACACTACCTAC |
| TDP | 58,261,468 | 58,261,491 | TTGCCAGTGTGAACCTGTAAGGCA |
| TDR | 58,261,526 | 58,261,545 | GCCACTGCTTGTTGCTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TTTGCTGACCACACCAGAAG |
| SD | TCTCCCTGAATGTCAGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13158A-A3 | VGB6 | C57BL/6NTac | |
| 13158A-A6 | VGB6 | C57BL/6NTac | |
| 13158A-E12 | VGB6 | C57BL/6NTac | |
| 13158A-G3 | VGB6 | C57BL/6NTac | |
| 13158A-G11 | VGB6 | C57BL/6NTac | |
| 13158A-G12 | VGB6 | C57BL/6NTac | |
