| Gene | Sft2d2 |
| Chromosome | 1 |
| Deletion Start | 165,194,299 |
| Deletion End | 165,179,178 |
| Deletion Size | 15,122 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 165,193,867 | 165,193,886 | GAGCCAGACCTTATGCGTTC |
| TUP | 165,193,824 | 165,193,851 | AAACTCCGTGAACGCCTGATAACAGGGA |
| TUR | 165,193,763 | 165,193,780 | CCGAGCGTGGTGAGAATC |
| TDF | 165,179,421 | 165,179,441 | GAACAGCCCTGGTGACTACAC |
| TDP | 165,179,393 | 165,179,417 | AATGATGTCATAGGCCCGACCTCCC |
| TDR | 165,179,324 | 165,179,345 | GGAAGGCCATGAGCACTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGTACTGCTCCAGCCTGTTG |
| SD | GGTCTGGGCAGAATCACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13164A-B9 | VGB6 | C57BL/6NTac | |
| 13164A-E5 | VGB6 | C57BL/6NTac | |
