| Gene | Slc35a3 |
| Chromosome | 3 |
| Deletion Start | 116,694,613 |
| Deletion End | 116,673,611 |
| Deletion Size | 21,003 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,694,200 | 116,694,223 | GACTTGAGTTTGGATCTTCAGCAC |
| TUP | 116,694,162 | 116,694,190 | TGGCTGCATATGGTAGTGTGAGTTTGTCA |
| TUR | 116,694,122 | 116,694,143 | GCTCCTGAGGTCCTCCTATTTC |
| TDF | 116,674,067 | 116,674,085 | TGTGCATGTCGGTGAGGTC |
| TDP | 116,674,040 | 116,674,065 | TGCACCATGGAGTGTAGGCTCAAAGC |
| TDR | 116,674,001 | 116,674,022 | GCCCAAATAGGTCGGAGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | AGTTGTTTGACGAGCGAACC |
| SD | TTTGCATGTGTGTGCTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13165B-G6 | VGB6 | C57BL/6NTac | |
| 13165B-C3 | VGB6 | C57BL/6NTac | |
