| Gene | 6330403K07Rik |
| Chromosome | 11 |
| Deletion Start | 71,033,399 |
| Deletion End | 71,032,986 |
| Deletion Size | 414 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,033,344 | 71,033,367 | TCCAGAGCAGATGTATTTCGATTC |
| TUP | 71,033,320 | 71,033,343 | TGACCCTGAGTACGAGGGCCTGTT |
| TUR | 71,033,258 | 71,033,278 | CAGCCGAGGATGCAGACTTAG |
| TDF | 71,033,160 | 71,033,180 | CCAGAGCCCAAGGAAGAAGAG |
| TDP | 71,033,135 | 71,033,158 | CTCCAGTGGAAGAGGGCTGCTACC |
| TDR | 71,033,093 | 71,033,112 | TGGCCGCGTAGATGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | GAGATAGACTTTGCTGCGCC |
| SD | CAGAAGGCTGGACTCTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13167E-D12 | VGB6 | C57BL/6NTac | |
