| Gene | Zdhhc11 |
| Chromosome | 13 |
| Deletion Start | 73,963,924 |
| Deletion End | 73,992,642 |
| Deletion Size | 28,719 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,963,945 | 73,963,967 | AGACACTAGCCAAAGGCAGTAAC |
| TUP | 73,963,970 | 73,963,998 | TCCAACTCATAACTCCCTGAATGAGAGCC |
| TUR | 73,964,009 | 73,964,028 | GTCTCCAGTGGGCTGAGTTC |
| TDF | 73,991,688 | 73,991,707 | GCACTCCCTGCATACTCCTG |
| TDP | 73,991,710 | 73,991,734 | CAGCCTCCTACATTAGCCTCCCGGT |
| TDR | 73,991,762 | 73,991,780 | CACATGCACCCAGCATCTC |
PCR Primers
| Primer | Sequence |
| SU | AAGTTGGCAGTGCACATCAG |
| SD | CCACAGACAACATATCAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13169A-A4 | VGB6 | C57BL/6NTac | |
| 13169A-H10 | VGB6 | C57BL/6NTac | |
| 13169A-C1 | VGB6 | C57BL/6NTac | |
| 13169A-E7 | VGB6 | C57BL/6NTac | |
| 13169A-D2 | VGB6 | C57BL/6NTac | |
| 13169A-B8 | VGB6 | C57BL/6NTac | |
