| Gene | 0610007L01Rik |
| Chromosome | 5 |
| Deletion Start | 130,229,471 |
| Deletion End | 130,241,335 |
| Deletion Size | 11,865 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,229,800 | 130,229,819 | TGCTGGGATTATGCGTTGTG |
| TUP | 130,229,823 | 130,229,852 | CACCACATCTGGACTGTTCTTTCTGAGACA |
| TUR | 130,229,860 | 130,229,878 | AGGGCAGCTTGGGCTTCAG |
| TDF | 130,240,633 | 130,240,653 | CACAGAAGTCATGCAGCAGAG |
| TDP | 130,240,655 | 130,240,683 | ACCTATCAGGGCCAACTAACTAGTGGCTA |
| TDR | 130,240,685 | 130,240,708 | CACTGTACTAACCAAGCCACATCC |
PCR Primers
| Primer | Sequence |
| SU | TTCTAGTCTTCCAAATGAGCTC |
| SD | AAGAACCACAGCAAACCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13171A-C5 | VGB6 | C57BL/6NTac | |
| 13171A-H6 | VGB6 | C57BL/6NTac | |
| 13171A-B11 | VGB6 | C57BL/6NTac | |
| 13171A-G4 | VGB6 | C57BL/6NTac | |
