| Gene | Gpc2 |
| Chromosome | 5 |
| Deletion Start | 138,279,811 |
| Deletion End | 138,274,221 |
| Deletion Size | 5,591 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,278,979 | 138,278,998 | GGTGAGCACCTTCAAGTCTG |
| TUP | 138,278,953 | 138,278,977 | CCTCAGGAGTACACCTGCTGTTCCA |
| TUR | 138,278,895 | 138,278,913 | TCTACCAGGCCACGGAATG |
| TDF | 138,274,426 | 138,274,447 | CTTGGAGTGAGAGGAGGAAGTG |
| TDP | 138,274,400 | 138,274,423 | AGTGCCAGATACAACCAGGGCCGA |
| TDR | 138,274,363 | 138,274,383 | GGGTATGGAGGCCAACAGATG |
PCR Primers
| Primer | Sequence |
| SU | TGCAATTGGCTCAGTTTCAC |
| SD | CAGGTTCTACCCTCCTTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13172A-C7 | VGB6 | C57BL/6NTac | |
| 13172A-C12 | VGB6 | C57BL/6NTac | |
| 13172A-A7 | VGB6 | C57BL/6NTac | |
