| Gene | Fndc3b |
| Chromosome | 3 |
| Deletion Start | 27,643,058 |
| Deletion End | 27,620,610 |
| Deletion Size | 22,449 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,642,625 | 27,642,643 | GCACAAGCCAAGGTGAGAC |
| TUP | 27,642,605 | 27,642,624 | AGCTCCTCTCCGCTGGCGGA |
| TUR | 27,642,558 | 27,642,579 | ACACAGGAGTGGCATCTGAAAG |
| TDF | 27,621,441 | 27,621,462 | CCTGACGTTTCAGTGTGGTAGC |
| TDP | 27,621,415 | 27,621,438 | AGGCCTCCTGCAAGAGCTAACACG |
| TDR | 27,621,364 | 27,621,385 | ACTGGCACTGTCATCCTGTCTG |
PCR Primers
| Primer | Sequence |
| SU | AAACGTGATCTCAGGGTCC |
| SD | TACCAGGTGAAGAGAACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13174A-G7 | VGB6 | C57BL/6NTac | |
| 13174C-A12 | VGB6 | C57BL/6NTac | |
| 13174C-E12 | VGB6 | C57BL/6NTac | |
