13175   Slc37a3     Status: ES cell colonies screened / QC positives

GeneSlc37a3
Chromosome6
Deletion Start39,366,974
Deletion End39,337,170
Deletion Size29,805
Genome Build38
MGI IDMGI:1919394
NCBI Gene ID72144
BAC ID290C4
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF39,366,92139,366,939CACCAGCTTCAGCCATCAC
TUP39,366,89639,366,920CACCTTGCTGTGTTCCTGCTCACGT
TUR39,366,87239,366,889TGTCAGCCAGCCATTACC
TDF39,338,03439,338,055GGCTAGGGATGCAGCATATTTG
TDP39,338,01139,338,033AGGCCCCAGAGTTCAGTCCCCAG
TDR39,337,98339,338,003TGAGCTGTCTCCTCTTTGTTC

PCR Primers

PrimerSequence
SUGTGTCAATAGGTCTGCTTTATG
SDAAAGGTGTGTTTCGTCCACC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR68 bp++-
TDFTDR73 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13175A-G11VGB6C57BL/6NTac
13175A-B12VGB6C57BL/6NTac
13175A-E2VGB6C57BL/6NTac
13175A-E1VGB6C57BL/6NTac
13175A-B8VGB6C57BL/6NTac
13175A-H6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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