| Gene | Lypd3 |
| Chromosome | 7 |
| Deletion Start | 24,636,593 |
| Deletion End | 24,640,660 |
| Deletion Size | 4,068 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,637,318 | 24,637,338 | GCCTCCTTAGGGACCTTTCAG |
| TUP | 24,637,341 | 24,637,364 | TCTGGCCACGCCTTTCCCTTTCAG |
| TUR | 24,637,371 | 24,637,388 | TGTGCAGCAGGGTCTCAG |
| TDF | 24,640,563 | 24,640,584 | GCAGTTCTGTTGACTGTGGTTG |
| TDP | 24,640,586 | 24,640,610 | TGGCGCGATGCTGTGAATGTCTCAT |
| TDR | 24,640,622 | 24,640,643 | GCCAGGGAAACAAAGTGAGATG |
PCR Primers
| Primer | Sequence |
| SU | ACCCTAGCAGACCCTGAATG |
| SD | TCCTGTGCCAGACAGAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13176A-E10 | VGB6 | C57BL/6NTac | |
| 13176A-B8 | VGB6 | C57BL/6NTac | |
| 13176A-C11 | VGB6 | C57BL/6NTac | |
| 13176A-E7 | VGB6 | C57BL/6NTac | |
| 13176A-F5 | VGB6 | C57BL/6NTac | |
