| Gene | 2700097O09Rik |
| Chromosome | 12 |
| Deletion Start | 55,080,074 |
| Deletion End | 55,045,771 |
| Deletion Size | 34,304 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,079,859 | 55,079,883 | CATACCCTACACCTAGTCATCAGAC |
| TUP | 55,079,802 | 55,079,830 | CAACTTTAGGTGGTTTGTGATTCCGGTGC |
| TUR | 55,079,743 | 55,079,766 | GTAGTATCGGCCTGTACGAGTAAT |
| TDF | 55,046,202 | 55,046,222 | GGAGCTGTCGAGATGGTTCAG |
| TDP | 55,046,178 | 55,046,201 | CCGCTTTAGAGCACAGGACCTGCA |
| TDR | 55,046,143 | 55,046,165 | AGCTGCGAACTGCCATATAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TGACCTTTGACCTTAGTGTTC |
| SD | CAGGAAGGGAAAGAACCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 141 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13179A-A10 | VGB6 | C57BL/6NTac | |
| 13179A-B7 | VGB6 | C57BL/6NTac | |
| 13179A-B11 | VGB6 | C57BL/6NTac | |
| 13179A-G2 | VGB6 | C57BL/6NTac | |
| 13179A-G10 | VGB6 | C57BL/6NTac | |
