| Gene | 3110056O03Rik |
| Chromosome | 10 |
| Deletion Start | 80,855,344 |
| Deletion End | 80,867,733 |
| Deletion Size | 12,390 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,856,091 | 80,856,108 | TGTGGCTCCAGCCTTCAG |
| TUP | 80,856,110 | 80,856,136 | CCCAGATCCTGGAGTGTTGCATCCTTG |
| TUR | 80,856,169 | 80,856,189 | GAGCGTACACAGTAGCAGGAC |
| TDF | 80,867,094 | 80,867,115 | GGCTTGGACTTGAGGGTTACTG |
| TDP | 80,867,119 | 80,867,145 | CCCACAGGATTTCACTGATGAGTGGGA |
| TDR | 80,867,221 | 80,867,241 | CCAGGGCTATGCAGCTTCATC |
PCR Primers
| Primer | Sequence |
| SU | TCATGTCACCTGGTCTGTAC |
| SD | GCTCTCAACTCCATCTTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 148 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13180A-B8 | VGB6 | C57BL/6NTac | |
| 13180A-B11 | VGB6 | C57BL/6NTac | |
| 13180A-C7 | VGB6 | C57BL/6NTac | |
| 13180A-E6 | VGB6 | C57BL/6NTac | |
| 13180A-G7 | VGB6 | C57BL/6NTac | |
| 13180A-F8 | VGB6 | C57BL/6NTac | |
