| Gene | Slc35b2 |
| Chromosome | 17 |
| Deletion Start | 45,564,719 |
| Deletion End | 45,567,294 |
| Deletion Size | 2,576 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,564,846 | 45,564,865 | GAGGGAGGCCAGTAAGTCAG |
| TUP | 45,564,873 | 45,564,892 | ATCGGGCTGGCTGCTCTGCA |
| TUR | 45,564,896 | 45,564,918 | AAGACACAGTCAAGGTCCACAAC |
| TDF | 45,566,368 | 45,566,389 | CACAGCCACATCACCAGGAGAG |
| TDP | 45,566,393 | 45,566,417 | TTCACAGACTCCCAGTTTCTGGTGC |
| TDR | 45,566,419 | 45,566,437 | CGCCAGCACACGGTTCATC |
PCR Primers
| Primer | Sequence |
| SU | GAGAAGGGAGGAGGTTCTGC |
| SD | CACAGCACCATTCTCACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13181A-B4 | VGB6 | C57BL/6NTac | |
| 13181A-B7 | VGB6 | C57BL/6NTac | |
