| Gene | Ranbp10 |
| Chromosome | 8 |
| Deletion Start | 105,827,265 |
| Deletion End | 105,805,538 |
| Deletion Size | 21,728 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,826,914 | 105,826,933 | CCGCTGGGAGACAAGTTTAC |
| TUP | 105,826,886 | 105,826,908 | CTGCAGGGAGGCTGTGCCCATTT |
| TUR | 105,826,832 | 105,826,850 | GACGTTCCGCTGGGCTAAG |
| TDF | 105,805,669 | 105,805,693 | GTCTGTGAAGGGAGATACAGCATAC |
| TDP | 105,805,638 | 105,805,664 | TAAGGAGTTTGATCAGGGCCACTCAGA |
| TDR | 105,805,594 | 105,805,617 | AGCAAACAGGGAGGGAATGAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CACTCTGGAGAAAGGCCAAG |
| SD | CAACTGCTAGGCCTTCTCTA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13182A-C2 | VGB6 | C57BL/6NTac | |
| 13182A-D3 | VGB6 | C57BL/6NTac | |
| 13182A-G8 | VGB6 | C57BL/6NTac | |
| 13182A-F4 | VGB6 | C57BL/6NTac | |
| 13182A-H12 | VGB6 | C57BL/6NTac | |
