| Gene | Bri3bp |
| Chromosome | 5 |
| Deletion Start | 125,441,665 |
| Deletion End | 125,454,769 |
| Deletion Size | 13,105 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 125,442,410 | 125,442,429 | CGTTTCCTCCCTGGTCTCTG |
| TUP | 125,442,439 | 125,442,465 | TTGTGGAGTCCAGAGAACTTTGGGTCC |
| TUR | 125,442,491 | 125,442,513 | GTCACCGACCTAATCTCATCCTG |
| TDF | 125,454,466 | 125,454,485 | GTGTTTGGCCGCTTCTTCTG |
| TDP | 125,454,488 | 125,454,511 | TCGTGCGTGTCATCCTGTTCTCCA |
| TDR | 125,454,532 | 125,454,551 | GGCTCGCCCTCGTACTTATG |
PCR Primers
| Primer | Sequence |
| SU | CGGAAATGCCATCTGTTCTC |
| SD | TTAAGGACGTCAAGCGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13192A-E6 | VGB6 | C57BL/6NTac | |
| 13192B-F3 | VGB6 | C57BL/6NTac | |
| 13192B-D6 | VGB6 | C57BL/6NTac | |
| 13192B-A6 | VGB6 | C57BL/6NTac | |
| 13192C-A4 | VGB6 | C57BL/6NTac | |
| 13192C-B2 | VGB6 | C57BL/6NTac | |
