| Gene | Lrrc52 |
| Chromosome | 1 |
| Deletion Start | 167,466,711 |
| Deletion End | 167,445,722 |
| Deletion Size | 20,990 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 167,466,257 | 167,466,276 | GCCAACACCACGTCTTTGAG |
| TUP | 167,466,228 | 167,466,253 | CCTGGACCTCAGAAACACCGGATTGC |
| TUR | 167,466,162 | 167,466,184 | GGTTCCCACTTAGGTACAGAGTC |
| TDF | 167,446,128 | 167,446,148 | CCGCCTGCTGTGTTTCTCTTC |
| TDP | 167,446,103 | 167,446,126 | CAGATGCCCAGAACGCCACGTGTA |
| TDR | 167,446,068 | 167,446,086 | CGTGATGGGCCACCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TGAGACCTTTCAGAGCTGCC |
| SD | GAATGTGGCCTCTTGCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 115 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13193A-H9 | VGB6 | C57BL/6NTac | |
| 13193A-B4 | VGB6 | C57BL/6NTac | |
| 13193A-A4 | VGB6 | C57BL/6NTac | |
| 13193A-E2 | VGB6 | C57BL/6NTac | |
