| Gene | Os9 |
| Chromosome | 10 |
| Deletion Start | 127,121,115 |
| Deletion End | 127,096,235 |
| Deletion Size | 24,881 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,120,549 | 127,120,568 | TCTTCCCGACCCTTCTTTCC |
| TUP | 127,120,522 | 127,120,547 | AGAGCCAAGCTTCAGACGTGGTGGTT |
| TUR | 127,120,477 | 127,120,498 | AGCTGGTAGGCGGCACTCATAG |
| TDF | 127,096,531 | 127,096,552 | TCGGCTGCCTACGTTTATAGAG |
| TDP | 127,096,508 | 127,096,530 | TCCCATCCTCATGTCCGAGGTGA |
| TDR | 127,096,448 | 127,096,470 | GCAGGGACTAGGGATATTGCTTC |
PCR Primers
| Primer | Sequence |
| SU | GTTTATTTCCGACAGCTGGG |
| SD | AACAGCAACAGGGAATGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13194A-H2 | VGB6 | C57BL/6NTac | |
| 13194A-D12 | VGB6 | C57BL/6NTac | |
| 13194A-B8 | VGB6 | C57BL/6NTac | |
