| Gene | Tspan3 |
| Chromosome | 9 |
| Deletion Start | 56,160,859 |
| Deletion End | 56,136,596 |
| Deletion Size | 24,264 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,160,485 | 56,160,502 | TCGGGTCCGCTGTCTTTG |
| TUP | 56,160,463 | 56,160,484 | TCTGAGTCCTCGCAGAGCCAGC |
| TUR | 56,160,359 | 56,160,378 | GCTTACCAGCCAACCAACTC |
| TDF | 56,137,082 | 56,137,103 | CAGTGGCACTGACTTAAAATGC |
| TDP | 56,137,053 | 56,137,077 | TAGACGTGAGTGCAGACACAAGCCA |
| TDR | 56,137,020 | 56,137,042 | CACTCCCCAAACCAGCTTCTATG |
PCR Primers
| Primer | Sequence |
| SU | AGGATTCTACACTCAAGGAC |
| SD | CGTCCTCCCTCTTTACCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 144 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13205A-A10 | VGB6 | C57BL/6NTac | |
| 13205A-B9 | VGB6 | C57BL/6NTac | |
