| Gene | Cldn12 |
| Chromosome | 5 |
| Deletion Start | 5,508,422 |
| Deletion End | 5,507,663 |
| Deletion Size | 760 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,508,228 | 5,508,248 | CGGTATGATGGAAGCAGTGAC |
| TUP | 5,508,204 | 5,508,227 | TGCCTGATGTACGACCGTACGTGG |
| TUR | 5,508,155 | 5,508,173 | GCAGGGCAAACTGGAGGAC |
| TDF | 5,507,834 | 5,507,856 | CCTGCTGTTCGTTTGGTATTGTG |
| TDP | 5,507,803 | 5,507,832 | ATGCAAGTCTTTGTCCTCTCCTTTCTGGCA |
| TDR | 5,507,749 | 5,507,772 | GATGAATAGGGCTGTGAGTAAGTG |
PCR Primers
| Primer | Sequence |
| SU | ATGTGTGCACTTGATCTGGG |
| SD | CTCCTAGCCTCATCCGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13208A-A6 | VGB6 | C57BL/6NTac | |
| 13208A-H5 | VGB6 | C57BL/6NTac | |
| 13208A-F12 | VGB6 | C57BL/6NTac | |
| 13208A-E2 | VGB6 | C57BL/6NTac | |
